Chemical Engineering of siRNA toward Modulating Endosomal Escape Kinetics

• Poly-dxC (exNA-dC) self-assembles into iMotif structures (ex-iM) specifically under the acidic pH condition
• Poly-dxC-modified siRNA self-assembles within the cell in vitro, enhancing the kinetics of siRNA activity without inducing toxic endosomal membrane rupture
• Poly-dxC-modified siRNA improves gene-silencing kinetics in vivo, with substantially enhanced early-time-point silencing in mouse kidney

Speakers:

Ken Yamada

Assistant Professor

University of Massachusetts Medical School